1) Transcriptional activation
转录激活
1.
Objective:To construct Smads transcriptional activation system for studying the function of Smads.
目的:构建Smads转录激活检测系统,以研究Smad2、Smad3、Smad4的功能。
2.
Methods: As EDAG has the activity of transcriptional activation, we set up a new model for large scaled screening small molecular compounds on 96-well plate by luciferase r.
方法:利用荧光素酶报道基因的实验方法,将EDAG的转录激活活性量化,建立大规模筛选活性小分子化合物的模型,筛选小分子化合物库(约1000个)。
2) transcription activation
转录激活
1.
The chromatin remodeling and histone acetylation were associated with transcription activation.
真核基因转录激活调控是在启动子、增强子等顺式调控元件和转录因子、激活因子以及 RNA聚合酶 II(Pol II)等调控蛋白的参与下协同进行的。
2.
In this study, a mammalian transcription activation system was constructed by using DNA binding domain(DBD) of GAL4 and luciferase reporter gene with DBD binding sequence, and used for mapping of FHL2 transcription activation domain.
利用GAL4转录因子中的DNA结合结构域 (DBD)和含有与DBD结合序列的荧光素酶报告基因(GAL4 LUC)构建了哺乳动物细胞转录激活系统 ,并利用该系统定位了FHL2的转录激活结构域。
4) Transcriptional activation activity
转录激活活性
1.
Transcriptional activation activity of variant was evaluated through methods .
方法:运用RT-nested PCR扩增人糖皮质激素受体(GR)基因不含编码LBD的cDNA序列,将PCR产物定向克隆至pEGFP-N2质粒载体,测序筛选编码正确的重组质粒;荧光显微镜观察GR突变体重组质粒转染人巨噬细胞株U937后的表达情况;相对荧光素酶法检测转染后细胞GR转录激活活性。
2.
The transcriptional activation activity of variant was evaluated by the relative activity of luciferase.
方法运用RT-nested PCR扩增糖皮质激素受体不含有编码LBD(ligand b ind ing dom ain)的cDNA序列,将PCR产物定向克隆至pEGFP-N2质粒载体,测序筛选重组质粒;荧光显微镜观察重组质粒转染表达情况;相对荧光素酶法检测GR转录激活活性。
3.
The transcriptional activation activity of GR was evaluated by the method of relative activity of luciferase.
方法 将GR荧光表达质粒pGFP GR转染大鼠肺泡巨噬细胞,经LPS和SNP作用后,荧光显微镜观察质粒表达产物GFP GR核移位情况;相对荧光素酶法检测GR转录激活活性;EMSA检测检测细胞NF κB活性。
5) transcriptional activation
转录激活活性
1.
Results showed that RDV P9 performed the transcriptional activation in yeast.
将RDV微核心蛋白基因S9克隆到酵母表达载体pGBKT7中,转入AH109酵母细胞,转化子可以在SD/Trp-His-Ade-多营养缺陷固体培养基上正常生长,证明RDV P9在酵母中具有转录激活活性,运用β-半乳糖苷酶的活性分析对重组质粒转化子的转录激活程度做了定量分析,发现与正对照相比,转化pGBK-S9的酵母菌中β-半乳糖苷酶活性可达到正对照的40%以上。
2.
In order to study the transcriptional activation activity of PC-1,firstly, the yeast two-hybrid system was used and the whole length as well as various regions of PC-1cDNA were cloned into the expression vector pAS2-1 and transfected into the yeast cell line CG-1945 respectively, the result of the reporter genes.
lacZ和His3报告基因激活的检测结果表明 ,该分子具有转录激活活性并将该活性定位于N端的 4 6个氨基酸区域 。
6) Activating transcription factors
转录激活因子类
补充资料：ρ转录终止因子
分子式：
CAS号：
性质：又称ρ转录终止因子。在转录终止时有特定效应的一种蛋白质。ρ因子能识别终止信号，防止RNA聚合酶继续抄录，并在模板上超出终点。可是，似乎有ρ-依赖的和ρ-不依赖的两者终端位点存在。
CAS号：
性质：又称ρ转录终止因子。在转录终止时有特定效应的一种蛋白质。ρ因子能识别终止信号，防止RNA聚合酶继续抄录，并在模板上超出终点。可是，似乎有ρ-依赖的和ρ-不依赖的两者终端位点存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条