1) SYBR Green I
SYBR GreenI
1.
Standard curve was established using a series dilution of quantified plasmids to measure EZH2 using SYBR Green I real-time fluorescent polymerase chain reaction and the characteristic of specific EZH2 amplicon was analysed by melti.
目的:建立SYBR GreenI实时荧光PCR定量检测人类EZH2基因的方法。
2.
According to the avian reticuloendotheliosis virus(REV) LTR sequence available in GenBank,a pair of specific primers were designed which target to the conserved region of LTR,and SYBR Green I-based real-time PCR was developed.
根据禽网状内皮组织增生症病毒(REV)长末端重复序列LTR基因保守区域设计并合成一对引物,建立基于SYBR GreenI模式的实时荧光PCR方法(Real-timePCR)。
2) SYBR Green I real-time PCR
SYBR~ GreenI实时PCR
3) Taq man
SYBR
1.
The HBVDNA in culture media were prepared with three methods for isolation and amplified by Taq man and SYBR real-time quatitiative PCR.
方法分别采用3种方法抽提HBVDNA,抽提物分别采用Taqman荧光定量、SYBR荧光定量PCR技术检测其滴度。
4) SYBR green Ⅰ
SYBR greenⅠ
1.
A rapid and sensitive Real-time PCR assay coupled with SYBR Green Ⅰchemistry was developed for the quantitative detection of Turbot reddish body iridovirus (TRBIV) isolated from farmed turbot (Scophthalmus maximus).
研究利用TRBIV主要衣壳蛋白基因序列设计的1对引物,结合内嵌式核酸染料SYBR GreenⅠ,建立了TRBIV特异的Real-timePCR检测方法。
5) SYBR dye
SYBR染料
6) SYBR GreenⅠ
SYBR Green Ⅰ
1.
Establishment of SYBR GreenⅠReal-Time Fluorescent Quantitative PCR for Detection of Duck Plague Virus;
SYBR Green Ⅰ实时定量PCR快速检测鸭瘟病毒的研究
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条