Cloning of variable region and signal peptide genes of anti-CD20 monoclonal antibody by RLM-RACE;

The RACE amplifying and sequenceanalysing of hepatocarcinoma highly expressed gene P02;

Cloning of a Pathogenesis-related Protein Gene cDNA of Potato Using RACE Methods Combined with cDNA Library;

METHODS: The full-length cDNA of rabbit BK_Ca beta subunit gene was amplified by 3′ RACE, 5′ RACE and RT-PCR from rabbit sphincter of Oddi (SO).

To find a novel gene which is related to protein of bovine seminal plasma (BSP) in human and study the new gene′s structural feature,expression and location in chromosome,the cDNA was cloned by using the 3′ rapid amplification of cDNA ends (RACE) and 5′ RACE methods and cloned into the expression vector of GST fusion protein (PGEX-5X-1),finally expressed in E.

Special primers were designed based on the sequence information of ferritin genes reported in the literature and used for rapid amplification of cDNA ends(RACE) for cloning Phascoloma esculenta ferritin gene.

To understand the mechanism of chick focal adhesion kinase gene(FAK)expression and regulation,the transcription start sites and four kinds alternatively spliced 5′end(5′untranslated region,5′UTR)of FAK mRNA in chick embryo fibroblast cells were identified by RACE.

Rapid amplification of cDNA ends (RACE) is a polymerase chain reaction (PCR)-based technique which was developed to facilitate the cloning of full-length cDNA 5 and 3 ends after a partial cDNA sequence has been obrained by other methods.

notoginseng,was amplified by RACE strategy with the total RNA of root as the template.

The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).

Gene specific primers derived from this partial sequence were used for the amplification of the 3′- and 5′-ends of this cDNA by RACE method,and this lead to a full-length cDNA sequence of 1 482 bp was amplified.