1) methylation-specific PCR
甲基化特异性PCR
1.
Establishment and preliminary application of methylation-specific PCR for detecting methylation of GTPase regulator associated with the focal adhesion kinase promoter
黏着斑激酶相关性鸟苷三磷酸酶调节因子基因启动子甲基化特异性PCR法的建立与初步应用
2.
Establishment and preliminary applification of methylation-specific PCR for detecting methylation of death-associated protein kinase promoter
死亡相关蛋白激酶基因启动子甲基化特异性PCR法的建立与初步应用
3.
Methods RT-PCR and Methylation-specific PCR (MSP) was used to detect the transcriptional expression and aberrant methylation of RASSF1A and p16 gene in 96 human NSCLC tissues and matched 96 normal lung tissues far from cancer area.
方法应用半定量RT-PCR和甲基化特异性PCR法分析96例NSCLC及远癌正常肺组织中RASSF1A和p16基因mRNA的表达和启动子区甲基化情况。
2) Methylation specific PCR
甲基化特异性PCR
1.
Methods The methylation status of CpG island of AR gene promoter in 3 leukemic cell lines and 15 leukemic bone marrow cells was detected by methylation specific PCR(MSP).
方法应用甲基化特异性PCR技术(methylation specific PCR,MSP)检测3种白血病细胞株及15例患者骨髓标本雄激素受体基因甲基化状态。
2.
The CpG island methylation status of DAPK was examined by means of methylation specific PCR(MSP),and the products of MSP were subjected to TA cloning.
方法收集40对新鲜的膀胱癌组织及其癌周正常组织;采用甲基化特异性PCR(methylation specific PCR,MSP)检测DAPK基因启动子区CpG岛的甲基化状态;并对MSP产物进行TA克隆。
3.
Methylation status was evaluated by methylation specific PCR,T-A cloning and sequence analysis.
方法用5- 杂氮-2’-脱氧胞苷对Hep-2人喉癌细胞系处理后,甲基化特异性PCR(MSP),T-A克隆测序分析死亡相关蛋白激酶(death-associ- ated protein kinase,DAPK)基因甲基化状态,逆转录聚合酶链反应、免疫细胞化学、流式细胞仪检测用药前后DAPK mRNA和蛋白的表达情况及细胞凋亡和周期的变化。
3) MSP
[英][,em es 'pi:] [美]['ɛm 'ɛs 'pi]
甲基化特异性PCR
1.
Methods 17 SCC and its surrounding normal lung tissues were chosed to study the INK4a/ARF methylation with the method of MSP(methylation-specific PCR).
方法收集外科手术切除标本SCC标本17例,同时选取周围正常肺组织作为对照,运用甲基化特异性PCR(MSP)方法对癌组织及其周围正常肺组织INK4a和ARF基因启动子的甲基化状态进行检测。
2.
Methods:Expression of DNMT1 mRNA was detected by in situ hybridization in 20 cases nomal cervical tissues, 60 cases CIN(CINⅠ,CINⅡ,CINⅢ,20 cases respectively);MSP was to determine the methylation status of CpG island in promoter region of E-cad.
方法:应用原位杂交技术检测20例正常宫颈、60例CIN(CINⅠ、CINⅡ、CINⅢ各20例)和40例宫颈鳞状细胞癌组织中DNMT1 mRNA的表达情况;利用甲基化特异性PCR(methylation specific polymerase chain reaction,MSP)方法检测E-cad基因启动子区甲基化状况,分析宫颈鳞状细胞癌中DNMT1 mRNA的表达与E-cad启动子区甲基化状况及2者之间的相关性。
3.
The methylation of CpG island in ER α gene promotor region A and B was respectively detected using methylation-specific PCR (MSP) and sequencing.
方法:采用免疫组化EnVisionTM二步法检测20例正常乳腺组织、44例乳腺癌组织ERα基因的表达,同时运用甲基化特异性PCR(Methylation-Specific PCR,MSP)及测序法分别检测ERα基因启动子A区和B区CpG岛的甲基化状态。
4) methylation special PCR
甲基化特异性PCR
1.
The methylation and unmethylation status of RASSF1A gene were detected by methylation special PCR(MSP)and RASSF1A mRNA expression was detected by RT-PCR.
材料与方法不同浓度的5-氮杂胞苷与Bcap-37细胞共培养后,应用细胞计数法检测Bcap-37细胞生长的情况;应用甲基化特异性PCR法(MethylationspecialPCR,MSP)检测5-氮杂胞苷作用前后RASSF1A基因甲基化、非甲基化状态的改变;采用逆转录PCR检测RASSF1A基因的mRNA表达。
5) MSP
[英][,em es 'pi:] [美]['ɛm 'ɛs 'pi]
特异性甲基化PCR
1.
Aim: To evaluate the clinical value of MSP(methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.
目的:评价特异性甲基化PCR(MSP)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。
6) MSP
[英][,em es 'pi:] [美]['ɛm 'ɛs 'pi]
甲基化特异性PCR(MSP)
补充资料：周期性特异性药物
周期性特异性药物
仅能杀灭*细胞增殖周期中某一期癌细胞的抗癌药物。依其作用又分为两类：①作用于S期药物，如*羟基脲、*阿糖胞苷，甲氨蝶呤、巯基嘌呤、硫鸟嘌呤等。其中前两种药物特异性较高，主要干扰DNA合成，但因也能干扰 RNA的合成，可能起到一定的自限作用。临床上宜缓慢静滴或肌注给药。②作用于M期的药物，如长春新碱等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条