Many related stimuli coming from intracellular or extracellular induce the activation of BH3(Bcl2-homology domains)-only protein,which regulates the formation of mitochondrial outmembrane permeabilization via members of Bcl-2 family,which results in the releasing of cytochrome c and Smac into the cytoplasm.

The expression of seven members of Bcl-2 family (including pro-apoptosis member of Bcl-2 family such as Bak, Bad, Bid, Bcl-xs and Bax, and anti-apoptosis ones such as Bcl-w and Bcl-2) and hepatitis B virus (HBV) proteins (such as, HBsAg, HBcAg and HbxAg) was detected in the tissues to texplore the significance of their expression.

Method Based on the immunohistochemical technology and electron microscope, We observed the relation between Bcl-2 family and the apoptosis in PC12 cells induced by dopamine.

The responses of TNF-R1 resulted in the activation of multiple apoptotic pathways involving the activation of the pro-death Bcl-2 family proteins,reactive oxygen species,c-Jun NH2-terminal kinase,ca- thepsin B,acidic sphingomyelinase and neutral sphingomyelinase.

The article discussed the roles of follicle-stimulating hormone,estrogen,androgen,stem cell factor,Bcl-2 family protein and p53 protein in differentiation and apoptosis.

AV-PIstaining was used for detecting apoptosis,PI staining was used for cell cycle analysis,and western blot was used for detecting caspase,BCL-2 family proteins and cell cycleregulating proteins.

Bcl-2 protein family and germ cells;

Bcl-2 and Bcl-xL proteins are main members of Bcl-2 protein family, which are new targets of anticancer drugs with bright prospect.

The role of Bcl-2 gene family in testis germ cell apoptosis;