1) RT-PCR
逆转录聚合酶连反应
1.
Methods Two siRNAs against the Her2 gene were designed and transfected into SK-BR-3 cells respectively, Her2 gene expression was measured using RT-PCR, Her2 protein expression was analysed using flow cytometer.
方法体外合成两条针对Her2/neu基因的siRNA,转染SK-BR-3细胞株;分别应用逆转录聚合酶连反应(RT-PCR)法和流式细胞仪测定Her2/neu基因和蛋白的表达,并用MTT比色法测定细胞的增殖活性,AnnexinV检测细胞凋亡。
2) Reverse transcription polymerase chain reaction
逆转录酶聚合酶链反应
3) Reverse transcriptase polymerase chain reaction
逆转录酶聚合酶链反应
4) reverse transcription polymerase chain reaction
逆转录聚合酶链反应
1.
[Methods] The level of human Fascin1 gene in endometrial carcinoma tissues(study group,n=30) and normal endometrial tissues(control group,n=10) was investigated by reverse transcription polymerase chain reaction.
方法选取30例新鲜子宫内膜癌组织标本(实验组)及10例正常子宫内膜组织标本(对照组),应用逆转录聚合酶链反应(RT-PCR)研究人类Fascin1基因的表达情况,并分析其与组织学类型、临床分期、病理分级、雌孕激素受体状态的相关性。
2.
After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).
二组分别于实验后1、4、7、10和14 d提取其肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定ATPase6、8 mRNA表达。
3.
Methods COX-2 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),and cell proliferation analyzed by cell counting kit-8(CCK-8) assay after exposure to dicl.
方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计数法测定药物作用72 h后的细胞增殖活性,绘制生长曲线。
5) Reverse transcription-polymerase chain reaction
逆转录-聚合酶链反应
1.
Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.
方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况。
2.
Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GRα and β mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.
方法采用逆转录-聚合酶链反应(RT-PCR)方法对18例GC治疗敏感、10例GC治疗抵抗的PM/DM患者PBMC中GRα、β mRNA的表达水平进行了检测,同时采用放射免疫分析法测定血清中的皮质醇含量,并与20例正常对照进行比较。
3.
Methods Using reverse transcription-polymerase chain reaction(RT-PCR),the PIM3 mRNA expression in normal murine ocular tissues was defected.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
6) Reverse transcriptase polymerase chain reaction
逆转录聚合酶链反应
1.
Methods The p24 gene fragment of BDV in PBMCs or CSFs in these patients infected in intracalvarium were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and .
方法用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)方法检测不明原因颅内感染患者及对照者PBMCs或CSFMCs中BDV p24基因片段,同时用β-肌动蛋白(-βactin)作为内参照,并总结阳性患者的临床特征。
2.
Methods: The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) in 52 patients with viral encephalitis and 30 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR.
方法:用荧光定量巢式逆转录聚合酶链反应(FQ-n RT-PCR)方法检测病毒性脑炎患者及正常体检者PB-MCs中BDVp24基因片段,同时用β-肌动蛋白(β-actin)作为内参照,总结出临床特征。
3.
The two consecutive fragments of human U5·116 ku were amplified by reverse transcriptase polymerase chain reaction (RT-PCR).
方法:从HeLa细胞中提取总体RNA,一步法合成单链cDNA,利用逆转录聚合酶链反应(RT-PCR)法,扩增出人类U5·116 ku基因两段连续的序列,首先分别克隆至pTZ57R/T载体,两段序列连接成全长后再定向克隆至真核表达载体pcDNA3。
补充资料：大目犍连《缁门崇行录》
【大目犍连《缁门崇行录》】
佛世大目犍连事母至孝，母死出家，精进行道，得六神通。见亡母生饿鬼中，持饭往饷，饭化猛火。目连痛哭白佛，佛言：“汝母罪重，非汝一人力所奈何。必假十方众僧威神之力，当于七月十五日佛欢喜日，僧自恣日，为母设盂兰盆斋，供佛及僧，始克济拔。”目连如教设斋，其母即以是日脱饿鬼苦，转更资荐，遂生天上。由此兰盆胜会流通万世焉。赞曰：“生养死葬，小孝也。生俾底豫，死俾流芳，大孝也。生导其正信，死荐其灵神，大孝之大孝也。目犍连以
之。”
佛世大目犍连事母至孝，母死出家，精进行道，得六神通。见亡母生饿鬼中，持饭往饷，饭化猛火。目连痛哭白佛，佛言：“汝母罪重，非汝一人力所奈何。必假十方众僧威神之力，当于七月十五日佛欢喜日，僧自恣日，为母设盂兰盆斋，供佛及僧，始克济拔。”目连如教设斋，其母即以是日脱饿鬼苦，转更资荐，遂生天上。由此兰盆胜会流通万世焉。赞曰：“生养死葬，小孝也。生俾底豫，死俾流芳，大孝也。生导其正信，死荐其灵神，大孝之大孝也。目犍连以
之。”
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条