Generation、Characterization of Anti-Hantaanvirus Glycoprotein Monoclonal Antibodies and the Expression and Identification of HTNV-G2 in the Pichia Pastoris Expression System;

Cloning and Expression of Akabane Virus Glycoprotein G1 Gene and Establishment of an Indirect Enzyme-Linked Immunosorbent Assay for Detection of Akabane Virus Antibodies;

To obtain a recombinant glycoprotein G1 of Akabane virus(AKAV)for diagnostic purpose,the gene fragment coding high antigenic domain of AKAV G1 protein was amplified from the AKAV genome by RT-PCR after analyzing glycoprotein G1 with bio-software.

Construction and transient expression of equine arteritis virus major envelope glycoprotein of eukaryotic expression vector;