1) DNA fragmentation
DNA片断
1.
Methods:The growth of SiHa cell in vitro was observed with different concentration extracts by MTT ;DNA cycle and apoptosis detected by flow cytometry;DNA fragmentation assay done and bax and caspase 3 checked by western blotting.
方法:观察不同浓度的不同蜈蚣提取物对宫颈癌SiHa细胞的生长抑制情况:运用体外细胞培养技术,以不同浓度的蜈蚣提取物和阳性对照药顺铂作用于培养的SiHa细胞48h,采用MTT法测定药物对细胞生长的影响,以流式细胞术观察DNA含量和凋亡的变化情况,DNA片断化分析凋亡特有DNA梯形格局和Western Blot测定bax、Caspase3蛋白水平的表达。
2.
DNA fragmentation assay was used to demonstrate effects of centipede extracts on SiHa cell line.
方法观察不同浓度的不同蜈蚣提取物对宫颈癌SiHa细胞的生长抑制情况:以不同浓度的蜈蚣提取物和顺铂作用于SiHa细胞48h,MTT法测定药物对细胞生长的影响,流式细胞术观察DNA含量和凋亡的改变,DNA片断化分析凋亡特有DNA梯形格局,Western blot测定bax、Caspase3蛋白水平的表达。
2) DNA Fragmentation
DNA片断化
1.
Methods In this study,a human normal liver cell line L-02 was treated with different concentrations of MCLR for toxicological investigation such as morphological changes, DNA fragmentation and mitochrondria membrane potential.
方法以不同浓度的微囊藻毒素LR(MCLR)处理L-02肝细胞,通过光镜和电镜下的形态观察、DNA片断化分析、线粒体膜电位变化等了解MCLR对肝细胞的毒性效应。
3) DNA fragmentation
DNA断片
1.
DNA fragmentation was visualized by agarose gel electrophoresis.
结果:TNF-α诱导BAEC产生典型的凋亡细胞形态学变化(核浓染,核碎裂)和DNA断片。
2.
DNA fragmentation was visualized by agarose gel elec.
结果:TNF-α5000kU/L和H_2O_2 300μmol/L均可诱导BAEC产生DNA断片。
3.
DNA fragmentation was visualized .
结果:TNF-α诱导BAEC产生典型的凋亡细胞形态学变化(核浓染,核碎裂)和DNA断片。
4) DNA fragment size
DNA断片大小
5) marker DNA fragment
标志DNA断片
6) DNA break
DNA断裂
1.
To evaluate the toxic effects of bisphenol A(BPA)on aquatic organism,different developed stages of zebrafish(embryo,larva and adult fish) were exposed to BPA solution,and the DNA break determination was done on zebrafish embryo.
结果显示:斑马鱼成鱼24h的LC50(8·00—10·00mg·l-1)比胚胎(16·4±0·40mg·l-1)低,表明斑马鱼成鱼更适用于急性毒性试验,而胚胎更能体现出亚急性效应和遗传效应;在5·00mg·l-1的BPA中连续暴露48h后,胚胎和成鱼体内的BPA含量分别为373·57±39·76μg·g-1和155·78±17·97μg·g-1,即胚胎在5·00mg·l-1BPA溶液中的蓄积能力大约是成鱼的两倍;DNA断裂检测表明BPA对斑马鱼胚胎的DNA造成了一定程度的损伤。
2.
Results:All three platinum-species drugs could cause DNA break of pBR322 plasmid.
方法 :用琼脂糖凝胶电泳和凝胶成像分析方法结果 :顺铂、卡铂、双环铂均可诱发 pBR322质粒DNA断裂 ,对质粒DNA的半数致断剂量分别为33。
补充资料：DNA分子花窗(沿B―DNA轴线方向看)
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条