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1)  stable transfection
稳定转染
1.
AIM: To construct recombinant eukaryotic expression vectors pEGFP-C3-SNCA containing human wild-type(WT) and pathogenic mutations A30P,A53T α-synuclein(SNCA),and to obtain monoclonal PC12 cell lines overexpressing human wild-type and pathogenic mutations A30P,A53T α-synuclein by stable transfection.
目的:构建含人野生型及致病突变A30P、A53TSNCA基因的重组真核表达载体pEGFP-C3-SNCA,并通过稳定转染获得过表达人野生型及致病突变A30P、A53Tα-synu-clein(SNCA)的单克隆PC12细胞株。
2.
[Objective] To investigate the effects on breast cancer cell line by stable transfection and analysis the cytobiology of the cells transfected by pcDNA3.
目的通过对乳腺癌细胞系T47D稳定转染,探讨真核表达载体质粒对细胞生物学特性的影响。
3.
The cell line, which express stably the encoding region and domain of M-CSF, respectively, was obtained by stable transfection o.
方法:PCR分别扩增人M-CSF的胞外活性区和功能区,连接到胞浆定位载体pCMV/cyto/myc质粒,得重组质粒pCMV/cyto/myc-h-M-CSF,再将重组质粒稳定转染NIH 3T3细胞,得到分别稳定表达M-CSF的胞外活性区和功能区的细胞株。
2)  The stably transfected cell
稳定转染株
3)  stable transfected CHO cell line
稳定转染的CHO细胞系
1.
Construction of eukaryotic expression vector of human MCHR2 and establishment of its stable transfected CHO cell line;
目的构建人MCHR2真核表达载体,转染CHO细胞,建立稳定转染的CHO细胞系。
4)  stable transfected SHG-44 cell line
稳定转染的SHG-44细胞系
5)  Stable transfected HepG2 cell lines
稳定转染的HepG2细胞系
6)  stably transfected SH-SY5Y cell line
稳定转染的SH-SY5Y细胞系
补充资料:世间转法转智转无尽
【世间转法转智转无尽】
  世间转者,谓展转摄前众生界、世界、虚空界也。法转者,谓展转摄前法界、涅槃界、佛出现界也。智转者,谓展转摄前如来智界,心所缘佛智所入境界也。盖此三转,皆言无尽者,以世法智之三种,展转含摄,无有穷尽,是名世间转、法转、智转无尽。
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