1) cold ethanol precipitation
冷乙醇沉淀
1.
HSA from plasma has long been produced by cold ethanol precipitation with purity about 96%, and final recovery 80%.
将层析技术与冷乙醇工艺相结合用于人血清白蛋白的纯化 ,对各过程所采用的层析介质及层析条件进行了探索 ,得到了一条从人血浆中制备血清白蛋白的新路线 :将一步冷乙醇沉淀后的血浆上清进行脱盐除乙醇 ,用阳离子交换介质CMSepharoseFF以透过式层析的模式吸附非白蛋白组分 ,最后选用ButylSepharoseFF一步疏水层析后所得样品经SDS PAGE银染显示一条单带 ,分析其纯度大于 99% ,计算工艺收率为 81 2 %。
2.
Fibrinogen was separated by cold ethanol precipitation and ion exchange chromatography in order to com-pare the effecy of separating fibrinogen.
为了比较冷乙醇沉淀法和离子交换层析法在分离止血胶成分中的纤维蛋白原的效果,本实验将灭活病毒的步骤加在溶解Nitschmann组分Ⅰ中进行,结果发现0。
2) ethyl alcohol precipitation
乙醇沉淀
1.
Immunoglobulin of egg yolk(IgY) can be extracted by the ice ethyl alcohol graduation centrifuge method and immunoglobulin can be purified gradually through the low temperature ethyl alcohol precipitation,Sephadex G-15 chromatography,A-50 ionic exchange chromatographic analysis,Tris-HCl buffer solution elution and Sephadex G200 gelatin filtration and so on.
通过低温乙醇沉淀、DEAE葡萄糖凝胶A-50离子交换层析、Tris-HCl缓冲液洗脱及Sephadex G200凝胶过滤等逐步纯化免疫球蛋白。
2.
The way of colloid adsorption together with ethyl alcohol precipitation isolated these two proteins successfully.
采用两性离子胶体沉淀和乙醇沉淀相结合的粗提方法,经离子交换、疏水层析、亲和层析及凝胶过滤 4个步骤有效地将人尿激肽原酶(hk 1)粗提物纯化,比活提高了 175 5倍,总得率为 70 %。
3) ethanol precipitation
乙醇沉淀
1.
MethodsThe following experiment were conducteds: pH control of fermentation,the concentration of ethanol for CPS extraction and nucleic acid precipitation,the temperature and time of ethanol precipitation and the times of protein extraction with cold phenol.
方法研究pH值,沉淀核酸和荚膜多糖的乙醇浓度,乙醇沉淀多糖所需的温度和时间;比较蛋白酶K法、Sevag法和冷酚抽提法去除荚膜多糖中杂蛋白质的效果。
2.
The experiment extracted mixed amino acids through acid hydrolysis, active carbon absorption and ethanol precipitation, and first studied the optimum extracting conditions of mixed amino acids from Capsella bursa-pastoris (L).
通过酸水解、活性炭吸附、乙醇沉淀进行氨基酸提取,首次确定了从荠菜提取混合氨基酸的最佳工艺条件。
3.
The method of ethanol precipitation together with ZnCl2 colloid precipitation was found to isolate themwith higher yield and activities.
本文通过ZnCl2胶体沉淀和乙醇沉淀相结合的方法可有效分离两者,得率高、活性保留好。
4) alcohol precipitation
乙醇沉淀
1.
The pumpkin pectin was obtained by alcohol precipitation after ultrafiltration.
本试验采用超滤浓缩后乙醇沉淀制备南瓜果胶。
2.
Extraction of soluble dietary fibre from soybean dregs by alcohol precipitation method
5 h条件下,采用碳酸钠溶液提取,滤液用4倍体积乙醇沉淀的方法提取可溶性膳食纤维(SDF),通过单因素实验和正交实验确定了碳酸钠溶液提取的最佳条件。
5) ethanol precipitation method
乙醇沉淀法
1.
The factors that influence the decolorizing effects of activated carbon were studied and the influence of ethanol precipitation method on product was discussed in the paper.
本文对影响活性炭脱色效果的几个因素进行了研究;并探讨了无水乙醇沉淀法对产品产率的影响,获得了活性炭脱色与无水乙醇沉淀的最佳工艺条件。
6) ethanol precipitation
乙醇沉淀法
1.
Pectin of orange bark was extracted by acid hydrolysis ethanol precipitation at different marinating time, pH and temperature conditions.
利用酸性水解乙醇沉淀法提取果胶,分别测定了不同的浸泡时间、酸碱度及水解温度对果胶产率的影响。
补充资料：冷沉淀反应
冷沉淀反应
在低温时发生的沉淀反应。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条