1) reversed paper chromatography
反相纸层析
2) reversed phase chromatography
反相层析
1.
6/250 reversed phase chromatography(RPC)were used for isolation and purification;SDS-PAGE was used to detect molecular weight(MW);Disc polyacrylamide gel eletrophoresis was used to measure the point of isoelectric(pI);Chromogenic substrate method was used to observe t.
方法应用苯甲脒-琼脂糖凝胶(Benzamidine Sepharose6B)亲和层析及Lichrospher C18反相层析柱进行分离纯化,应用SDS-PAGE测定分子量、聚丙烯酰胺凝胶盘状电泳测定等电点,以发色底物法测定生物活性。
3) normal and reversed-phase chromatography
正反相层析
4) reversed phase extraction chromatography
反相萃取层析
1.
Using chlorazotic acid[φ(chlorazotic acid)=20%] as mobile phase,with tri butyl phosphate on sillca gel as the stationary phase,Gold(Ⅲ) and Thallium(Ⅲ) can be separated from Fe 3+ 、Cu 2+ 、Ag + 、Zn 2+ 、Mn 2+ 、Hg 2+ 、Cd 2+ 、Pb 2+ 、Sb 5+ 、As 5+ 、Ga 3+ 、In 3+ 、Pt 2+ 、Pd 2+ etc by reversed phase extraction chromatography.
以王水（体积分数为２０％）作移动相，以硅胶球负载的ＴＢＰ作固定相，反相萃取层析使微量Ａｕ（Ⅲ）、Ｔｌ（Ⅲ）与Ｆｅ３＋、Ｃｕ２＋、Ａｇ＋、Ｚｎ２＋、Ｍｎ２＋、Ｈｇ２＋、Ｃｄ２＋、Ｐｂ２＋、Ｓｂ５＋、Ａｓ５＋、Ｇａ３＋、Ｉｎ３＋、Ｐｔ２＋、Ｐｄ２＋等多种离子分离，留于柱上的铊、金分别用ＮａＡｃ溶液（量浓度为１ｍｏｌ／Ｌ）及Ｎａ２ＳＯ３溶液（质量浓度为１０ｇ／Ｌ）洗脱进行连续分离与测定（结晶紫光度法测定铊，孔雀绿光度法测定金），回收率可达９６％以上，方法已应用于地质样品中金、铊的连续分离与测定。
6) paper chromatography
纸层析法
1.
Methods Under the various separating condition,the anticoagulating active components of scorpion were appraised with thin layer chromatography(TLC) and paper chromatography.
方法用不同的展开剂,采用薄层色谱法和纸层析法对全蝎抗凝活性成分进行鉴定和分析。
2.
0 mg of ascorbic acid; A labeled efficiency more than 97% is obtained by paper chromatography.
0 mg; 纸层析法分析标记物, 标记率大于97%。
3.
Objective To estabilsh a paper chromatography method for analyzing radiochemical purity of cyclic DTPA anhydride coupled anti-CEA monoclonal antibody (McAb) labeled with 153 Sm.
目的 :应用纸层析法 ,建立153Sm通过DTPA环酐 (cDTPAa)标记抗CEA单抗的放射化学纯度分析方法。
补充资料：防水相纸
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性质： 见涂塑相纸
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