Study on protein expression and cell transformation of novel cadmium related gene TIF3;

This paper studies the carcinogenicity of five kinds of diesel exhaust particulates(DEPs),by using SHE cell transformation assay and Unscheduled DNA Synthesis Test(UDS Test).

MATERIAL AND METHODS: We used the improved BALB/c-3T3 cell transformation assay to observe the transformation of cells treated by: GEN, B[a]P(10μmol/L) + GEN and MCA(0.

Results One line of nickel sulfide transformed cells and the related tumor cell were found to contain a mutation in exon 8 in P53 gene.

[ Objective ]To determine if the DNA damage such as DNA strand breaks and DNA-protein crosslinks are present in transformed cells induced by three nickel compounds, and to further elucidate the relative potential carcinogenesis of nickel compounds with different solubility.

Proteomics Analysis Reveals Insight into the Mechanism of H-Ras-Mediated Transformation;

The previous studies have shown that transfection of NIH3T3 cells with a novel cadmium related gene TIF3 (GenBank Accession Number AF271072) resulted in overexpression of its encoded protein inducing NIH3T3 cell transformation when transfected.

Bacground: The bovine papillomavirus type 1 (BPV-1) E6 oncoprotein induces tumorigenic transformation of murinc C127 cells and stimulates transcription when targeted to a promoter.