1) high performance hydrophobic interaction chromatography
高效疏水色谱
1.
The effects of composition of mobile phase,stationary phase,pH and gradient mode for high performance hydrophobic interaction chromatography(HPHIC) on the separation of recombinant bovine mature PrP c highly expressed in E.
在此基础上 ,确定了牛朊病毒正常成熟蛋白分离纯化的最优化条件 ,即仅用高效疏水色谱法进一步便可得到纯度和质量回收率均大于 90 %的目标蛋白 。
2.
Reduced and denatured RNase A by using high performance hydrophobic interaction chromatography(HPHIC) was studied.
用高效疏水色谱(HPHIC)研究了还原变性核糖核酸酶(RNase A)在HIC上流动相中脲浓度、流动相流速及蛋白浓度对还原变性RNase A复性的影响。
2) high performance hydrophobic interaction chromatography(HPHIC)
高效疏水作用色谱法
3) high performance hydrophobic interaction chromatography
高效疏水作用色谱
1.
A protocol comprising PEG8000 precipitation, high performance hydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) for separation and purification of cytochrome P450 in the cotton bollworm, Helicoverpa armigera, was introduced.
作者建立了适用于棉铃虫中肠微粒体P450的纯化方法,包括聚乙二醇8000(PEG8000)沉淀、高效疏水作用色谱(HPHIC)和高效离子交换色谱(HPIEC)等连续分离步骤。
4) hydrophobic interaction chromatography
疏水色谱
1.
A simple-type column of hydrophobic interaction chromatography packed with big particles was employed for the fast purification in laboratory scale.
使用一种装有大颗粒疏水填料的简易型疏水色谱柱在实验室规模对细胞色素C进行了纯化 ,通过反相色谱、紫外光谱及铁含量对样品的纯度进行了检验。
2.
The refolding of redcuced- denatured insulin from bovine pancreas on the hydrophobic surface was studied with high performance hydrophobic interaction chromatography ( HPHIC) .
用疏水色谱 ( HPHIC)对还原变性的牛胰岛素在疏水界面上的折叠规律进行了研究 ,对于未还原的胍变和脲变 insulin来说 ,胍变 insulin用 HPHIC法可以完全复性 ,而脲变 insulin只能部分复性。
3.
Purification of the cytotoxic fraction by gel filtration chromatography on Superdex 75 and hydrophobic interaction chromatography on Phenyl Sepharose HP respectively.
结果眼镜蛇毒粗毒经凝胶过滤获得 4个蛋白峰 ,将CTX所在第Ⅳ峰用阳离子交换柱色谱获A、B、C、D、E、F和G等7个组分 ,其中E、F和G具CTX活性 ,将F组分再经凝胶过滤和疏水色谱进一步纯化得不含磷酯酶A2 (PLA2 )的CTX纯品 ,暂定名为CTX F ,它对多种癌细胞株有杀伤作用。
5) hydrophobic chromatography
疏水色谱(法)
6) hydrophobic interaction chromatography
疏水作用色谱
1.
Almost all the lipid in fresh hemolysate of bovine red blood was removed with hydrophobic interaction chromatography (HIC) in the presence of 2%PEG4000, 5%PEG4000, 2%PEG10000 or 5%PEG10000.
经过疏水作用色谱后 ,血红蛋白的P5 0是 3386 4Pa,Hill系数是 2 。
2.
AIM: To investigate the refolding of the reduced/denatured ribonuclease (RNase) by high performance hydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) and to study the factors influencing its refolding.
目的:研究还原变性的核糖核酸酶(RNase)在高效疏水作用色谱(HPHIC)及高效离子交换色谱(HPIEC)上的复性及影响因素。
补充资料：高效薄层色谱
分子式：
CAS号：
性质：使用5～10μm的硅胶微粒制备的薄层色谱板。采用程序多级展开技术，将常量薄层色谱的灵敏度和分辨力大大提高。在一块10×20cm板上能检出十几个至30个样品。分析时，大致有以下几个步骤：吸附剂及薄层板的制备、点样、展开和定量等。高效薄层色谱的优点：容量大，效率高、快速，一块板上能对40个样品进行分离，平均每分钟分离5个以上。定量准确、灵敏，能检出10-9～10-12g的物质。
CAS号：
性质：使用5～10μm的硅胶微粒制备的薄层色谱板。采用程序多级展开技术，将常量薄层色谱的灵敏度和分辨力大大提高。在一块10×20cm板上能检出十几个至30个样品。分析时，大致有以下几个步骤：吸附剂及薄层板的制备、点样、展开和定量等。高效薄层色谱的优点：容量大，效率高、快速，一块板上能对40个样品进行分离，平均每分钟分离5个以上。定量准确、灵敏，能检出10-9～10-12g的物质。
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参考词条