Based on a comparative study of two coating methods, with mycoplasm hyopneumoniae (antigen) or its antibody, a highly sensitive and accurate enzyme linked immunosorbent assay (ELISA) method has been developed for direct determination of biotin in the transformed yeast cultural media.

Method: Mycoplasm hyopneumoniae was used to coat the microtiter plate and its antibody in rabbit and goat anti-rabbit IgG incorporated biotin were linked to the plate successively to form a fixed phase of biotin which, after optimization of the experimental conditions, was applied in the followed competition inhibition reaction to determine bio.

Preparation and identification of monoclonal antibodies against HSP70 of Mycoplasma hyopneumoniae;

Expression of Dnak-P97 fusion gene of Mycoplasma hyopneumoniae and biological activities of the expressed product;

Development of monoclonal antibodies against Mycoplasma hyopneumoniae;

Pulmonary Mucormycosis in 13 Cases and Review of Literature

Prokaryotic expression of Actinobacillus pleuropneumoniae out membrane lipoprotein gene;

Prokaryotic expression of capsular polysaccharide genes of Actinobacillus pleuropneumoniae and cytotoxicity assay of expressed products to porcine neutrophil;

Detection of Actinobacillus pleuropneumoniae by PCR;

Cross Immunoprotection of Actinobacillus pleuropeumoniae Serotypes 1 and 5;

A pair of primers from the 5′ and 3′ termini of outer membrane lipoprotein(oml) genes of Actinobacillus pleuropeumoniae(App) were selected to be the basis for delvelopment of a specific PCR assay.