1) two-dimensional electrophoresis
双向电泳
1.
Comparative study of three thymic protein refining methods in two-dimensional electrophoresis;
3种方法提取胸腺组织蛋白双向电泳纯化结果比较
2.
Mechanism of hepatocyte transformation by HCV NS3 using two-dimensional electrophoresis and mass spectrometry;
双向电泳和质谱技术分析HCV NS3转化肝细胞的蛋白质组
3.
Analysis of two-dimensional electrophoresis maps of congenital total cataractous and normal lens proteins;
先天性全白内障晶状体和透明晶状体蛋白双向电泳差异比较
2) two-dimensional gel electrophoresis
双向电泳
1.
Development of two-dimensional gel electrophoresis profiles of proteomics from GSF cells;
GSFs细胞蛋白质组双向电泳图谱的建立
2.
Establishment and preliminary analysis of the two-dimensional gel electrophoresis map of neural retinal proteome in rats;
SD大鼠视网膜蛋白质组双向电泳图谱建立和初步分析
3.
Two-dimensional gel electrophoresis of proteome in human pancreatic juice.;
人胰液蛋白质组的双向电泳
3) 2-DE
双向电泳
1.
Analysis of HSP70 protein polymorphism with 2-DE and Western-blot;
人淋巴细胞HSP70蛋白多态性双向电泳-Western blot分析
2.
The establishment of the two dimensional gel electrophoresis (2-DE) map of hypertrophic scar epidermal cells and the display of the differentially expressed proteins;
增生性瘢痕表皮细胞双向电泳图谱的建立及差异蛋白展示
3.
Preliminary establishment of two-dimensional gel electrophoresis(2-DE) for muscle tissue of bloody clam Tegillarca granosa;
泥蚶肌肉组织蛋白质双向电泳体系的建立
4) two dimensional electrophoresis
双向电泳
1.
Proteome of human CD4~+CD25~+T cells vs CD4~+CD25~-T cells: Comparative analysis by two dimensional electrophoresis;
人CD4~+CD25~+T细胞和CD4~+CD25~-T细胞蛋白质组的双向电泳分析
2.
The application of two dimensional electrophoresis in the separation of proteins in Bombyx mori;
双向电泳法在家蚕蛋白质分离中的应用
3.
Here,we present a method for the proteomic analysis of CNB sample based on the two dimensional electrophoresis.
本文建立和优化了适合针刺活检样品的基于双向电泳的蛋白质组学分析平台:通过直接抽提获得针刺活检组织的蛋白质样品;用24cm固定梯度干胶条(pH3-10NL)等电聚焦及12。
5) 2-D electrophoresis
双向电泳
1.
An analysis of 2-D electrophoresis and immunoblotting of 18.6kD protein antigen of Fasciola hepatica;
肝片吸虫18.6kD蛋白抗原双向电泳及免疫印迹分析
2.
Objective For elucidating the molecular mechanism of reconstruction of intestinal epithelium damaged by ionizing radiation,the proteomes of murine intestinal epithelium from normal and irradiated mice were compared by 2-D electrophoresis.
分离未照射小鼠及照射后 3h和 72h小鼠的肠上皮细胞 ,制备蛋白样品 ,采用双向电泳技术分离样品 ,PDQuest软件处理双向电泳图谱 ;并对差异的蛋白质点进行肽质量指纹分析。
3.
In order to research relative proteins with grain plumpness during filling stage of rice,one serial of 2-D electrophoresis which be suitable to endosperm proteins of rice seed was set up.
为探讨水稻灌浆期间与籽粒充实相关蛋白表达的变化,建立了一套适于水稻种子胚乳蛋白双向电泳分析技术,探讨了胚乳蛋白的提取、纯化以及双向电泳的一些关键技术。
6) Two-dimensional electrophoresis(2-DE)
双向电泳
1.
During the two-dimensional electrophoresis(2-DE),the first dimension electrophoresis was performed on a 7 cm strip with pH 3-10 linear immobilized pH gradient(IPG)and the second one was performed on 12.
为了探索适用于双向电泳(2-DE)分析的苹果叶片蛋白质提取方法,比较了三氯乙酸(TCA)/丙酮沉淀法、二硫苏糖醇(DTT)/丙酮法、Tris-HCl提取法和改良的Tris-HCl提取法等4种蛋白质提取方法。
2.
Methods:The total proteins of root were extracted with trichloroacetic acid(TCA)/acetone precipitation method and ultracentrifugation,and then separated by two-dimensional electrophoresis(2-DE),which was an IPGphor TM unit with immobile pH gradient strips used in and the Ettan TM Dalt Ⅱ system in SDS-PAGE.
目的:建立温莪术的双向电泳技术(2-DE)体系。
3.
To characterize an unknown estrogen-responsive protein ULF-250,which was first identified from the uterine luminal fluid(ULF) of rat in this laboratory,the ULF was collected from the estradiol-treated ovariectomized rats,and protein components of the ULF were separated by SDS-PAGE and two-dimensional electrophoresis(2-DE).
收集ULF分别进行SDS-PAGE和双向电泳(2-DE)分离;通过Western印迹和2D-Western确认抗ULF-250抗体识别的蛋白成分并与凝胶上的条带或斑点相对应;对目标蛋白分别用MALDI-TOF-MS和HPLC-ESI-MS/MS两种方法进行质谱分析并获得肽段序列数据;与蛋白数据库比对及文献检索鉴定未知蛋白。
补充资料：双向电泳
分子式：
CAS号：
性质：以等电聚焦作为第一向，将蛋白质按等电点不同分开，然后将已按等电电点分开的蛋白质进行SDS-聚丙烯酰胺凝胶电泳作为第二向，让等电点相同或相近的蛋白质再按分子量大小分开。双向电泳使分辨率大大提高，若结合同位素标记等技术，分辨率将会更高，可将多蛋白组分中等电点或分子量相同或相近的蛋白质分开。
CAS号：
性质：以等电聚焦作为第一向，将蛋白质按等电点不同分开，然后将已按等电电点分开的蛋白质进行SDS-聚丙烯酰胺凝胶电泳作为第二向，让等电点相同或相近的蛋白质再按分子量大小分开。双向电泳使分辨率大大提高，若结合同位素标记等技术，分辨率将会更高，可将多蛋白组分中等电点或分子量相同或相近的蛋白质分开。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条