The Michaelis constant and activation energy of the enzymatical reaction were also determined,which are in good agreement with previously reported values.

Km,the Michaelis constant of chitinase,is 24.

Through the relation between the reaction rate and the concentration of substrate, the determinate Michaelis constant Km of the polyphenol oxidase (PPO) of barley was 1.

Measurement of Km(NADH) of LDH from Rabbit Muscle;

g) and Km of F1 .

Aim To explore the effects of solanine on Km and Vmax of NATase of HepG2.

The apparent kinetic parameters(apparent Michaelis-Menten constant,K_(mapp),and apparent maximal reaction rate,V_(mapp)) were estimated by nonlinear fitting reaction curve to the integrated Michaelis-Menten rate equation with the predictor variable of reaction time.

METHODS: Uricase reaction was monitored by the absorbance at 293 nm and apparent kinetic parameters (apparent Michaelis-Menten constant, K_ mapp, and apparent maximal reaction rate, V_ mapp) were estimated based on the calibrated specific acti.

A spectrophotometric assay of the tyrosinase activity was used to determine the kinetic parameters of K_m and V_(max) by monitoring the initial rate of formation of dopachrome at 475 nm.

0 and under relatively high-temperature condition;the enzymic activity was activated by NH_4~+,and strongly inhibited by Pb~(2+),Fe~(3+);the apparent Michaelis-Menten constant(Kmapp)of the reaction was 0.

A systematical measure of the polarity scale of the Chinese porous polymer beads of this series of stationary phase has been carried out, including the McReynolds constant at 120 ℃ by using squalane as non-polar stationary phase and the relative constant to GDX-103 at 180 ℃.