1) Mannanase gene
甘露聚糖酶基因
1.
A plasmid ,pMD-18Tman2XcmI is developed,the plasmid is constructed by cloning an Xcm?I cassette with mannanase gene (man 1?101?bp long) between two Xcm?I restriction sites into pMD-18T.
通过克隆甘露聚糖酶基因到pMD-18T载体构建了质粒pMD-18Tman2XcmI,克隆的甘露聚糖酶基因两侧各引进了一个特定的XcmI酶切位点;用XcmI酶切质粒pMD-18Tman2XcmI可以制备T载体。
2) β-mannanase gene
β-甘露聚糖酶基因
1.
Using a pair of degenerated primers,the conserved fragments ofβ-mannanase gene from the selected strains were amplified by PCR.
将基因片段测序并同已发表的β-甘露聚糖酶基因进行相似性分析,并构建进化树。
3) Mannanase
甘露聚糖酶
1.
Recombination of mannanase gene man23 and its expression in Brevibacillus brevis;
甘露聚糖酶man23基因的重组及其在短短芽孢杆菌中的表达
2.
Expression of recombinant mannanase gene Man23 in Bacillus sp. WB600;
甘露聚糖酶基因Man23在芽孢杆菌WB600中的表达
3.
Construction of a double functional recombinant strainof Pichia pastoris co-expressing phytase and mannanase and the enzymatic analyses;
植酸酶和甘露聚糖酶双功能毕赤酵母工程菌的构建和产酶分析
4) Mannosyl transferase
甘露糖基转移酶基因
5) PMI gene
磷酸甘露糖异构酶基因
1.
The PMI gene was transferred to the expression vector pBI-121, which allowed the selection of transgenic plants with mannose as selective agent.
根据GENBANK数据库中大肠杆菌(Escheriachia coli)的磷酸甘露糖异构酶基因(phosphomannose isomerase PMI)序列设计引物,通过PCR的方法得到约1200bp片段并与克隆载体pGM-T easy连接,经测序证明与已发表PMI序列同源性达到100%。
6) β-mannanase
β-甘露聚糖酶
1.
Study on the immobilized β-mannanase by chitin;
甲壳素固定化β-甘露聚糖酶的研究
2.
Study on the Production of β-mannanase by Bacillus M-21;
芽孢杆菌M-21产β-甘露聚糖酶发酵条件研究
3.
Breeding of β-mannanase Producing Strains;
β-甘露聚糖酶产生菌的选育
补充资料：糖酶
分子式：
分子量：
CAS号：
性质：能使糖在水解的酶的总称。例如淀粉酶、转化酶、麦芽糖酶、溶菌酶等。
分子量：
CAS号：
性质：能使糖在水解的酶的总称。例如淀粉酶、转化酶、麦芽糖酶、溶菌酶等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条