METHODS: SP immunohistochemical staining and immunofluorescence were performed in paraformaldehyde fixed hippocampal sections.

METHODS Indirect immunofluorescence method and MTT method were used to test the expression of human IL-6 receptor on R2 cells and the reaction of R2 cells to recombinant human IL-6(rhIL-6).

Methods Immunohistochemistry was used to detect expression of ABCG2 in 40 cases of transitional cell carcinoma of bladder with different pathology grades(12 cases were Grade Ⅰ,18 cases were Grade Ⅱ,10 cases were Grade Ⅲ)and 15 normal bladder tissues,while immunofluorescence was used to detect expression of ABCG2 in BIU-87 cell line.

Expression of Survivin in leukemia by Western blot analysis and immunofluorescence method;

RuBPCase in leaf blade transection of typical C 3, C 4 plants and Hevea brasiliensis was located by direct immunofluorescence method.

Rabbit antiserum raised purified ribulose 1,5 bisphosphate carboxylase (RuBPCase) from tobacco was used to locate RuBPCase in leaf blade transection of classical C 3, C 4 plants and Coffea arabica by direct immunofluorescence method.

Application of indirect immunofluorescence(IFL) in the detect of antibody to eberthella typhi and bacillus paratyphosus;

Methods: The ANA was detected with indirect immunofluorescence(IIF) and protein chip respectively,and the other six autoantibodies including anti-dsDNA,anti-Ro-60/SSA,anti-Ro-52/SSA,anti-SSB,anti-Jo-1,and anti-phospholipid IgG were simultaneously detected with ELISA and protein chip technology,and numbers of the both positive,both negative and the si.

METHODS: Balb/c mice was immunized with pig cardiac myosin and complete freund ,s adjuvant(CFA), β 1 integrin on myocardium were analyzed by laser confocal microscopy and indirect immunofluorescence at the day 14,30,60 after the first immunization.