The reaction can be realized by enzymatic or non-enzymatic catalysis or by combination of two methods.

In order to improve the catalytic properties of enzymes,the author and his group have devoted themselves to the researches of enzymatic molecular modification,enzyme immobilization,non-aqueous enzymatic catalysis and directed enzyme evolution in the past two decades,finding that the catalytic properties of enzymes such as activity and stability.

This experiment focused on the synthesis of 1,4-dipalmitoyl hydroxyproline by Schotten Banmamm condensation reactions, and studied the non-aqueous enzyme catalysis.

Optical resolution of racemic meta-substituted inherently chiral aminocalix[4]arenes has been effectively realized via the nonenzymatic kinetic resolution method,utilizing Boc-L-proline and Cbz-L-proline as chiral acylating reagents.

Results show that Phanerochaete chrysosporium has special abilities of enzyme catalysis biodegradation of PAM.

Results Applications including enzyme catalysis in SCF fermentation in SCF,SCF sterilization and cell disruption were reviewed.

The technology of ultra-microparticle by SCF、enzyme catalysis in supercritical fluid and supercritical water oxidation were introduced,their applications were also elaborated.