1) random mutation
随机突变
1.
In vitro molecular directed evolution of enzymeis a new strategy in the engineering of novel biocatalysts, which mainly mimics the process of natural evolution to generaterandom mutation in the genes coding for useful enzymes by the techniques of error-prone PCR, and then in vitro recombinespositive mutations through various methods such .
酶的体外定向进化是改造生物催化剂的一种有效的新策略,主要是模拟自然进化进程,通过容错PCR等技术对编码酶的基因进行随机突变,再由DNA改组、交错延伸过程、随机引导重组、递增截短技术等进行突变基因的体外重组,最终经筛选获得所需的酶。
2.
Several receptor binding sites of LT were mutated randomly, then the R46,S106,L130 combined site-directed random mutation library and S106～F110 region random mutation library were displayed on a filamentous phage surface.
对LT的受体结合区域进行多点随机突变,利用噬菌体展示重组人淋巴毒素(rhLT) R46,S106,L130三点随机突变组合文库和S106～F110区域随机突变体库。
3.
To construct the combined site_directed random mutation library of recombinant human Lymphotoxin (rhLT) for in vitro molecular evolution study, and to study the structure and function relationship.
应用含随机核苷酸序列的引物 ,通过OverlapPCR的方法分别对rhLT的 4 6、10 6和 130位氨基酸进行定点随机突变 ,获得各单点随机突变体库。
2) random mutagenesis
随机突变
1.
Many research groups successfully rely on whole-gene random mutagenesis and recombination approaches for the directed evolution of enzymes.
许多研究小组利用整基因随机突变形成和重组方法,已成功的进行了酶的定向进化。
3) random site-lirected mutation
随机定点突变
4) site-directed random mutation
定点随机突变
1.
Objective: To construct a phage-displayed site-directed random mutation combinatorial library of immunoglobμLin-binding molecμLes, which can provide reference the study of the in vitro molecμLar evolution of the library with different immunoglobμLins,and the relationship of their structure and function.
目的:构建免疫球蛋白结合分子定点随机突变组合噬菌体展示文库,为应用各种类型免疫球蛋白对该文库进行体外分子进化筛选及研究免疫球蛋白结合分子结构和功能打下基础。
5) random mutant library
随机突变文库
1.
We have established TCS random mutant library, and therefore, it facilitates theprokary.
本实验利用PCR技术从栝楼基因组DNA中扩增出TCS基因，成功构建了克隆载体和表达载体，并建立了TCS随机突变文库，这为TCS的原核表达奠定了分子生物学的基础。
6) random mutagenesis library
随机突变体库
1.
Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.
目的:通过构建酵母ARS随机突变体库,从中筛选出具有较强的转化效率的ARS序列,用以构建新型高效的克鲁维酵母基因工程载体。
补充资料：随机数和伪随机数
随机数和伪随机数
random and pseudo-randan numbers
随机数和伪随机数【喇间佣1 al川牌”山一喇闭..m.山娜;cJI了，a如曰e”nce，口oc月卿成.以叹“c月a】 数亡。(特别，二进制数:。)，其顺序出现，满足某种统计正则性(见概率论(probability Uleory)).人们是这样区别随机数(mndomn切mbe比)和伪随机数(PSeudo一mn由mn切mbe岛)的，前者由随机的装置来生成，而后者是用算术算法构造的.总是假设(出于较好或较差的理由)所得(或所构造)的序列具有频率性质，这些性质对于具有分布函数F(z)的某随机变量心独立实现的一个序列来说是“典型的”;因此人们称作根据规律F(习分布的(独立的)随机数.最经常使用的例子为:在区间【O，l]上均匀分布的随机数亡。，尸(亡。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条