1) Gel filtration chromatography
凝胶过滤层析
1.
Ultrafiltrations(UF) with 1 kD membrane and gel filtration chromatography(GFC) with Sephadex G-15 were used to separate the hydrolysates into different components.
使用1kD超滤膜对水解液进行超滤,超滤液采用葡聚糖凝胶Sephadex G-15凝胶过滤层析得到多种组分。
2.
Objective To find out the optimal condition for separation of the protein mixture by gel filtration chromatography.
目的 探讨凝胶过滤层析分离蛋白质的最佳实验效果。
3.
Two major allergens PT 13 and PT 53 in platanus hispanica pollen were isolated and purified by ion exchange chromatography and gel filtration chromatography.
以离子交换和凝胶过滤层析对梧桐花粉变应原进行分离和纯化。
2) gel filtration
凝胶过滤层析
1.
Methods: A novel reverse co immunoprecipitation method was used to get the purified BTV HbC particles and gel filtration, electron microscopy(EM), TCID 50 and plaque assay was used to analyse the purification results.
方法 :利用反向免疫共沉淀方法获取纯化的蓝舌病毒颗粒 ;运用透射电镜和凝胶过滤层析检测纯化产物病毒的结构完整性和纯度 ;组织培养技术检测产物的生物学活性并计算纯化得率。
2.
Cholinesterase from duck serum was purified to electrophoretic homogeneity by salting out twice with ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-200.
2磷酸盐缓冲溶液等体积稀释后,以硫酸铵为盐析剂进行二次盐析、再经DEAE-52离子交换层析和Sephadex G-200凝胶过滤层析对胆碱酯酶进行分离纯化,可获得电泳纯的胆碱酯酶,纯化倍数为278。
3.
Crude laccase was collected after fermentation broth was filtrated with vacuum pump,and then purified to electrophoretic homogeneity by the steps of ammonium sulfate precipitation,ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephadex G-75.
将发酵液经真空抽滤后得粗酶液,以硫酸铵为盐析剂进行分级盐析沉淀、再经DEAE-52离子交换层析和Sephadex G-75凝胶过滤层析对漆酶进行分离纯化,用SDS-PAGE证明可获得纯的漆酶,纯化倍数为183。
3) gel-filtration chromatography
凝胶过滤层析
1.
Optimization of the gel-filtration chromatography parameters for separating proteins from the crude extracts of housefly;
凝胶过滤层析参数对家蝇蛋白粗提液分离效果的影响
2.
Then,BMP was isolated and purified by the methods of D301 ion-exchange chromatography(IEC)and Sephadex G-25 gel-filtration chromatography(GFC).
使用反相高效液相色谱(RP-HPLC)对重组酵母发酵液中一种牛肉风味肽(BMP)的稳定性进行了研究,并分别运用D301离子交换层析和Sephadex G-25凝胶过滤层析对其进行了分离纯化,最后利用RP-HPLC对目标肽进行分析检测。
3.
β-Lactoglobulin was purified from whey protein concentrate by a combination of Ammonium sulfate precipitation and gel-filtration chromatography .
通过SDS-聚丙烯酰胺凝胶电泳检测蛋白成分,结果显示:①在20%～30%、70%～80%盐析段只有两条带,分别是β-乳球蛋白和α-乳清白蛋白带,得到较纯的蛋白;②再用SephadexG-50凝胶过滤层析,电泳结果显示只有一条带,并与标准β-乳球蛋白带一致,得到较纯的β-乳球蛋白。
4) Gel permeation chromatography
凝胶过滤层析
1.
Xylobiose and xylotriose were prepared from xylooligosaccharides by gel permeation chromatography,and their in vitro cultivation properties by Bifidobacterium adolescentis were investigated.
采用凝胶过滤层析技术获得了高纯度木二糖和木三糖,并研究了它们在体外对青春双歧杆菌的增殖效果。
2.
The discipline of enzymatic hydrolysate of xylooligosacchardes(XOS) separation with polyacrylamide gel was investigated, and high purity of homogeneous XOS were obtained by using a established preparative gel permeation chromatography system.
本文研究了聚丙烯酰胺凝胶分离低聚木糖的规律;首次建立了大量制备分离低聚木糖单一组分的凝胶过滤层析系统,获得了可用作分析标准品的木二糖和木三糖;首次采用体外间歇厌氧培养的方式,对青春双歧杆菌代谢低聚木糖及低聚木糖单一组分的规律和机理进行了研究。
5) gel chromatography
凝胶过滤层析
1.
Then, the antibacterial crude extracts were purified using Sephadex G 75 gel chromatography and two steps CM Sepharose ion exchange chromatography.
将经过上述处理的粗提液经过一步 Sephadex G-75凝胶过滤层析、两步 CM-Sepharose阳离子交换层析后 ,得到一个单一的具有抗阳性球菌的峰 。
6) gel filtration chromatography
凝胶过滤柱层析
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条